Which processing event occurs to create the primary transcript by adding a 5' cap and a 3' poly-A tail?

Which processing event occurs to create the primary transcript by adding a 5' cap and a 3' poly-A tail?

• A. Translation
• B. Splicing
• C. 5' cap added to transcript and 3' poly-A added to transcript
• D. Reverse transcription

Answer: (C)

RNA processing of eukaryotic pre-mRNA includes adding a 5' cap and a 3' poly-A tail to produce mature mRNA. The 5' cap is a modified guanine that protects the transcript and helps ribosomes recognize and initiate translation. The poly-A tail added at the 3' end increases stability and assists with export from the nucleus. Splicing is another processing step that removes introns, but it is a separate modification from capping and tailing. Translation uses the mature mRNA to synthesize protein, while reverse transcription converts RNA back into DNA and is not part of normal mRNA maturation.

RNA processing of eukaryotic pre-mRNA includes adding a 5' cap and a 3' poly-A tail to produce mature mRNA. The 5' cap is a modified guanine that protects the transcript and helps ribosomes recognize and initiate translation. The poly-A tail added at the 3' end increases stability and assists with export from the nucleus. Splicing is another processing step that removes introns, but it is a separate modification from capping and tailing. Translation uses the mature mRNA to synthesize protein, while reverse transcription converts RNA back into DNA and is not part of normal mRNA maturation.